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KMID : 0624620130460010053
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BMB Reports
2013 Volume.46 No. 1 p.53 ~ p.58
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Biochemical characteristics of functional domains using feline foamy virus integrase mutants
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Yoo Gwi-Woong
Shin Cha-Gyun
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Abstract
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We constructed deletion mutants and seven point mutants by polymerase chain reaction to investigate the specificity of feline foamy virus integrase functional domains. Complementation reactions were performed for three enzymatic activities such as 3'-end processing, strand transfer, and disintegration. The complementation reactions with deletion mutants showed several activities for 3'-end processing and strand transfer. The conserved central domain and the combination of the N-terminal or C-terminal domains increased disintegration activity significantly. In the complementation reactions between deletion and point mutants, the combination between D107V and deletion mutants revealed 3'-end processing activities, but the combination with others did not have any activity, including strand transfer activities. Disintegration activity increased evenly, except the combination with glutamic acid 200. These results suggest that an intact central domain mediates enzymatic activities but fails to show these activities in the absence of the N-terminal or C-terminal domains.
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KEYWORD
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Complementation reaction, Deletion mutant, Feline foamy virus, Integrase, Point mutant
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